With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). 313 reference gain check fail. Measure oxidative metabolism and glycolytic activity on the SpectraMax i3x reader. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. SpectraMax i3x Multi-Mode Detection Platform User Guide. , 2014; Xiong et al. 3: The cuvette chamber. The plate was shaken and incubated in the dark for 10 minutes. Fluorescence Microplate Reader Comparison Chart. If desired, a high-range standard curve may be prepared from 1 ng/mL to 1 μg/mL, or a low-range standard curve may be prepared from 25 pg/mL to 25 ng/mL. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. 306 plate air cal. infoboxeu@moldev. Spectranet customer portalSpectraMax i3x Multi-Mode Microplate Reader with MiniMax Imaging Cytometer by Molecular Devices® Manufacturer Molecular Devices® | Model: i3x | Available Worldwide 4. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. It allows you to explore cellular pathways and protein activation and expression in one system. SpectraMax ® iD3 and iD5 Multi-Mode . Bound crystal violet was dissolved with 10% acetic acid and the optical density (OD) at 570 nm was determined using a Spectra Max i3X spectrophotometer (Molecular Devices). SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. These concentrations are then used to perform western blots. Ferguson, Ph. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email drugdiscoverytech@lifetech. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. SpectraMax i3x, onboard luminescence 121. It allows you to explore cellular pathways and protein activation and expression in one system. The Molecular Devices SpectraMax i3X Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence. ZERO BIAS - scores, article reviews, protocol conditions and more. Formicro-volumemeasurements,theinstrumentsupportsSpectraDrop24-wellSpectraMax i3 Install Guide; SpectraMax i3; SpectraMax i3x; SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Features technology from the SpectraMax i3x and MiniMax 300 Imaging Cytometer. 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. Prismatic colour and motion for everyone. The plate was covered and incubated for one hour at room temperature. Learn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. 303 unable to cal dark current. Molecular Devices are known for both the reliability and the wide uses of their products. Category 2 and 3 microplate readers use gradient heating as well. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Description 1. 2 x 10-14. • SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . The CellTiter-Glo assay from Promega uses luciferase. Cet appareil ISM est confomre à la norme NMB-001 du Canada. The Molecular Devices SpectraMax i3x Microplate Reader is a multi-mode microplate reader, able to measure UV/vis, fluorescence and luminescence. Using the SpectraMax i3x reader, the preconfigured SpectraMax Glo Steady-Luc Reporter assay protocol in SoftMax Pro Software was used to measure luminescence. Table 1. Home > Search Results > Danaher Inc > spectramax i3x. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. pathways and protein activation and expression in one system. If desired, a high-range standard curve may be prepared from 1 ng/mL to 1 μg/mL, or a low-range standard curve may be prepared from 25 pg/mL to 25 ng/mL. The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. Pick the best speed, sensitivity, and price for your needs. 在SpectraMax i3x和SpectraMax iD5微孔板读板机上使用 SoftMax Pro软件绘制标准曲线，并进行4参数曲线拟合(图 4)，结果见表2。使用EC50评估该测定方法的适用性，EC50值 应低于150 nM。对于两个读板机，EC 50值均低于85 nM，处 于可接受范围内。Cisbio将可接受的信噪比定义为≥. The assay window spanned four orders of magnitude. Hands-on fun and learning that empowers exploring, inventing and sharing. Register to download your free eBook today. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. Danaher Inc spectramax i3x. The SpectraMax® i3 Platform from Molecular Devices® is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. 02, 0. The SpectraMax i3x reader provides theThe SpectraMax® i3x Multi-Mode Microplate Reader with the SpectraMax® MiniMax™ 300 Imaging Cytometer can be used to image slides using a specialized slide adapter. Find regional support. Features. User-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. When used in combination with the SpectraMax Injector Cartridge, this system enables a wealth of flash type luminescence applications including reporter gene and other assays with exceptional sensitivity and dynamic range. The SpectraMax ® i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The TRF intensities measured are proportional to the level of extracellular acidification in each sample. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. 3 In this application note we demonstrate a linear dynamic range of 6 decades for recombinant firefly and Renilla luciferases, as well as linear detection of luciferase-transfected. ATP (“flash” luminescence using . See SpectraMax® Microplate Reader User Manual Download Page. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Prime function 20 amol. Molecular Devices社の製品: 吸収度マルチモードマイクロプレートリーダー SpectraMax® i3x に関するすべての情報をご覧ください。. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. com. Robust,. Be Future Ready with the SpectraMax i3x Multi-Mode Detection Platform. Find regional support. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. In luminescence mode, if you are specifying a wavelength for the read, you can select an emission bandwidth of either 15 nm or 25 nm. With optimized reagents, option to operate the readers. Get a front row view of your cells. Molecular Devices’ ScanLater Western Blot Detection Cartridge uses TRF detection to turn its SpectraMax i3X and Paradigm plate readers into Western blot readers. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellThe absorbance was recorded on a spectra Max i3x microplate reader (Molecular Devices, USA) at 450 nm. 2. The SpectraMax® i3 Platform's base system features an integrated optical system enabling top and bottom reads for 6-, 384- well microplates and launches with three broad detection modes: luminescence, absorbance, and fluorescence. detection cartridge LUM96: 59. The soil suspension (600 µL) and the substrate (L-3,4-dihydroxyphenylalanine, DOPA, 150 uL, 25 mmol L -1 ), were mixed and then added to. A: percent GFP-positive cells calculated using the classification feature in the software. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. 5. The SpectraMax i3 also allows the user to identify antibodies during Elisa Assays. D. SpectraMax i3/i3x readers must be equipped with a suitableWhen we measured the FRET ratio using the Spectramax i3x plate reader, we found that FRET ratios were higher in DMSO-treated cells that had not been incubated at room temperature to enhance fluorescence than in room temperature-incubated cells (Fig. Ideal for a wide range of applications, including Western Blot analysis, this SpectraMax i3x includes the additional MiniMax 3000 Imaging Cytometer module for advanced cell imaging. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. The absorbance at 450 nm was read with a Spectra Max i3X microplate spectrophotometer (Molecular Devices, Sunnyvale, CA, USA), and the data at 30–40 min post-treatment were analyzed. Benefits • User-upgradeable application modules including cellular imaging • Sensitivity across spectrum with Spectral Fusion™ Illumination • Expanded dynamic range with cooled PMT • Control and analytics provided by SoftMax® Pro Software SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. SpectraMax iD5:Fluorescence was quantified using a microplate reader (SpectraMax i3x, Molecular Devices, CA, USA) with an excitation and emission wavelength being 365 nm and 450 nm, respectively (Jian et al. High resolution X-ray photoelectron spectroscopy (XPS) of cCQD was obtained on a K-Alpha+ (Thermo Fisher Scientific, Massachusetts, USA) spectrometer. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the dataBased on the SpectraMax i3x system, ACROBiosystems provides biomedical research and development services for customers seeking quantification and detection of antibodies-antigen interaction. Order Now. 7 mm for all shake intensities. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. 吸収度マイクロプレートリーダー SpectraMax® Mini. The MPO assay was conducted according to the. MD Neurotransmitter Transporter Assay. The SpectraMax DuoLuc Reporter Assay has been performance optimized on the SpectraMax i3x reader with Injector Cartridge. See Unpacking the SpectraMax Paradigm Instrument on page 1. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular. Then, the cell area was calculated for each. , Fisher Scienti fi c, cat. However, no piece of equipment is immune to breaking down. +44 118 944 8000. Sunnyvale, CA, USA) with excitation at 475 nm. The dual auto injector module. Unpacking and setting up the instrument includes the following procedures: • Unpacking the instrument and saving the original packaging. The resulting absorbance was measured at 490 nm, Spectra Max i3x (Molecular Devices, USA). Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Patented user-exchangeable cartridge design expands the system's detection capability making it. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. GFP expression was higher at 21 hours post transfection than at 16 hours (16-hour data not shown). NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. Promega. Capabilities include fluorescence and luminescence readings, environmental control, cell viability assays, enzyme. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Spectramax I3x, supplied by Danaher Inc, used in various techniques. Contact Us Phone: +1. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,*On the SpectraMax i3x reader only. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. The LDH activity in the cultural media was measured based on the reaction between LDH and lactic acid, which led to the generation of pyruvic acid, and pyruvic acid could become brown in alkaline environments with 2,4. Read height should be optimized for . Increased fluorescence performance. comThe SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening SoftMax® Pro 7. . SpectraMax® i3x Multi-Mode Microplate Reader with MiniMax™ imaging cytometer Integrated multi-mode microplate reader and imaging system • Ideal for cell counting, label-free imaging, and cell proliferation • Western blot, imaging, and injectors on one configurable microplate reader • Stain free, automated cell countingusing the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. Superior flexibility The SpectraMax i3 Multi-Mode Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. 00800 665 32860. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Video: SpectraMax i3x Overview; Brochure: SpectraMax i3x Multi-Mode Detection PlatformNORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. ZERO BIAS - scores, article reviews, protocol conditions and moreWe would like to show you a description here but the site won’t allow us. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the datathe SpectraMax i3x Multi-Mode Microplate Reader with SpectraMax Injector Cartridge Introduction Reporter gene assays are used to study the expression of eukaryotic genes. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. Adding time resolved fluorescence, HTRF® or western blot detection simply requires the user to exchange a detection module. Enzyme activity was presented as absolute activity in a unit of nmol g −1 dry soil h −1. 7°C and the bottom to 36. The Relative Luminescence Units (RLU) were measured using the SpectraMax i3x Luminescence Glow cartridge (Lum 384) (0200-7015POS, Molecular Devices) and normalized to the cell count or area, which were determined using the Mini MaxTM 300 Imaging Cytometer (5024062, Molecular Devices). The calculated LOQ values were 0. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. This instrument also provides the option of modular upgrades for Western Blotting,. Contact our support team with your questions regarding Molecular Devices products and applications. Spectranet customer portal The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. SpectraMax® i3 Multi-ModeDetectionPlatform withtheSpectraMax® MiniMax™ImagingCytometer UserGuide 5025027A April2013. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,i3x Multi-Mode Microplate Reader. Reduce background noise The cooled PMT reduces background noise allowing for a more sensitive, wide dynamic range in extremely low light. *On the SpectraMax i3x reader only. Detection of pro-inflammatory factors. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the. Average 98 stars, based on 1 article reviews. The assay window spanned four orders of magnitude. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. ). Devices instruments including: SpectraMax®, VersaMax, VMax®, EMax®, Gemini, ThermoMax, and FlexStation® readers. We would like to show you a description here but the site won’t allow us. Download PDF. The cell imaging was captured on Zeiss LSM 980 confocal microscopy. 蛍光 発光 ELISA. Find regional support. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). Remove the transport bracket and store the retaining screws in the bag that held the 3. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Published Dec 16, 2020 | Updated Oct 10, 2023. 0. Examples: fiPress carefully to engage,fl fiDo not press down. Fluorescence Reader. Supplier: Molecular Devices. Myeloperoxidase (MPO) Assay. Devices instruments including: SpectraMax®, VersaMax, VMax®, EMax®, Gemini, ThermoMax, and FlexStation® readers. SeeSpectraMax MiniMaxImagingCytometeronpage85. (SpectraMax i3/i3x) CatchPoint SimpleStep ELISA (SpectraMax Paradigm) SpectraDrop. 312 gain calibration failed. For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. ; India imports most of its Spectramax from United States, China and Austria and is the largest importer of Spectramax in the World. SpectraMax i3x 多功能微孔板读板机 SpectraMax Paradigm 多功能微孔板读板机 SpectraMax M5e 多功能微孔板读板机 扫一扫关注我 们的官方微信 Molecular Devices 大中华区 全国咨询服务热线: 400-820-3586 Email: info. Table 1. 2. Data Analysis. 1. Example: [Drawer]. Eliminate time-dependent substrate addition steps. Fluorescence was measured using a microplate fluorometer (SpectraMax i3x, Molecular Devices, Beckman Coulter, CA, USA) with 365 nm excitation and 450 nm emission filters. 0. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. 0´ 104 1. We would like to show you a description here but the site won’t allow us. applications, including dual luciferase and ATP assays. 발광 (Luminescence) 리더기. Time-. In SoftMax® Pro, the following Automix settings are available for the SpectraMax i3/i3x Instrument: Intensity : Low, Medium, or High. Prepare your i3/i3x instrument for shipment. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. 0 mm hex key to remove the six blue retaining screws that secure the transport bracket (B) to the instrument. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. 9 x 10-15. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. BRET2canbeusedtostudyvariouskindsofprotein-proteininteractions. 314 low lamp level warning. Itemploysa luciferaselabel(where“label”generallymeansfusedbytheuseofexpressionvectors)forthe oneproteinandaGreenFluorescentProtein(GFP)variantlabelfortheother. , 2021). Explore a wealth of applications in one future-ready system that measures spectral-based absorbance, fluorescence, and luminescence. Structured Review. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence. flThe SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. The information below applies to a standard 96 well plate, set to a linear shake mode. Pick the best speed, sensitivity, and price for your needs. Time-resolved fluorescence was measured on the SpectraMax Multi-Mode Microplate Readers (see Table 2 for instrument settings). The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. Measured using the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. The corrected absorbance is within 5% of the. For more detailed information and technical support of Molecular Devices instruments or software,The SpectraMax® Paradigm Multi- Mode Microplate Reader with Tunable Wavelength (TUNE) Detection Cartridge from Molecular Devices combines the flexibility of wavelength scanning with the high sensitivity of filters. 吸収度マイクロプレートリーダー SpectraMax® Mini. The SPECTRAmax 340PC can mea- Learn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. Native or oxidized AN (40 μM) and FN (0. It allows you to explore cellular pathways and protein activation and expression in one system. This way you’ll save time on finding the necessary info. 1. 2. The ABS reader ranges from 340-850 nm in wavelength and the ABS Plus reader ranges from 190-1000 nm, which is equivalent to having 510 or 810 free filters, respectively. 0´ 104 8. i3x. The chemical reduction of resazurin was evaluated incubating each well of the microtiter plate with 10 μL (10% of final well volume) of 0. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. Molecular Devicesの全商品を見る. Relative mitochondrial polarization was expressed as the ratio of emission at 590 nm to that at 530 nm (Shin et al. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strengthThe SpectraMax i3x reader grows with you as your research areas expand. Table 3 Optimal instrument settings for Valita Titer Plus assay Fluorescence Polarization measurement on Molecular Devices microplate readers. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Next, an AlphaLISA assay was developed to detect levels of pro-inflammatory factors produced by endothelial cells in response to TNF-alpha stimulation. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. 2/5 apart, not needed o Otherwise, ensure cutoff is 10nm less than desired emission wavelength. After incubation at 37 °C and 5% CO 2 for 20 min, fluorescence images were taken using a Spectra Max i3x with MiniMax 300 Imaging Cytometer (Molecular Devices, San Jose, CA, USA) with the excitation and emission setup provided with SoftMax Pro 7. applications, including dual luciferase and ATP assays. 価格、見積もり、お近くの販売店を. Prepare your i3/i3x instrument for shipment. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Figure 4. 99. Cell viability was calculated as average percent of the OD reading relative to that of control group, which was normalized to 100%. Replicate values were averaged and imported into Design-Expert 8. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. techsupport. 8 Antibodies and. Molecular Devices, LLC Reading Modes: Endpoint and Kinetic; Optical System: Spectral Fusion Illumination. Generate more data points without the need to dilute. 2. • SpectraMax i3x • Onboard luminescence • Detection cartridge (LUM 96) • SpectraMax iD5 • SpectraMax L • SpectraMax M5e • SpectraMax Mini. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology:. 1 mg/mL density of resazurin, protected of the light, incubated for 1 h at room temperature. SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence and luminescence. Learn how the SpectraMax Paradigm Multi-Mode Detection Platform with Tunable Wavelength (TUNE) Detection Cartridge combines the flexibility of wavelength scanning with the high sensitivity of filters. 1step assay with SpectraMax microplate readers. When the user applies a set temperature of 37°C, the top is heated to 37. Spectranet customer portalThe SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. 모든 제품. SpectraMax i3x 多功能微孔板读板机 SpectraMax Paradigm 多功能微孔板读板机 SpectraMax M5e 多功能微孔板读板机 扫一扫关注我 们的官方微信 Molecular Devices 大中华区 全国咨询服务热线: 400-820-3586 Email: info. The treatments were. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. 2. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Cell counters for accurate and. SpectraMax i3x and Paradigm readers require the FP-FLUO detection cartridge. SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. Example: [Drawer]. SpectraDrop Abs Protein Quant (i3-i3x)Molecular Devices SpectraMax i3x Multi-Mode Detection Platform. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. 0 | 9 reviews | Write your own review Measures Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgradesMolecular Devices SpectraMax i3x Multi-Mode Microplate Platform. Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. Thetypeof. com The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Spectranet customer portalWe would like to show you a description here but the site won’t allow us. The difference in absorbance observed in the presence and absence of SOD was considered to be the amount of superoxide produced, and results. Overview of SpectraMax MiniMax 300 Imaging Cytometer. Manufacturers try to design their instruments so they can be. 3, were mixed in a final volume of 100 μL in 10 mM sodium phosphate buffer, pH 7. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Modularity does provide some measure of forward vision into upcoming or future experimental needs. 001 0. After correcting for negative controls and quench standards, the soil enzyme activities were expressed in units of nmol h −1 g −1 soil (German et al. ) wide and 65 cm (25. VAL014, right table) assays on the SpectraMax i3x reader are shown below. The assay can accurately test the toxicology of compounds as shown in Figure 1. The TUNE detection cartridge uses a breakthrough monochromator design that enables researchers to easily optimize their. 06, 0. Description 1. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3, i3x and Paradigm Multi-Mode Readers. Besides, it’s possible to examine each page of the guide singly by using the scroll bar. Biocompare is the leading resource for up-to-date product information, product reviews, and new technologies for life scientists. Introduction. Table 1. Figure 4. SpectraMax i3x. Benefits • User-upgradeable application modules including cellular imaging • Sensitivity across spectrum with Spectral Fusion™ Illumination • Expanded dynamic range with cooled PMT • Control and analytics provided by SoftMax® Pro Software SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves. Transfected cells were imaged on the SpectraMax MiniMax 300 Imaging Cytometer. 3) At the bottom of the strip, click on "Show. The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3, i3x and Paradigm Multi-Mode Readers. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your intial investment. spectramax i3x (Danaher Inc)The fluorescence emission intensity spectra were recorded using a microplate reader (Spectra Max® i3x, Molecular Devices, LLC. 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . The SpectraMax® i3x offers a wealth of applications; it can measure fluorescence, absorbance and luminescence, and its modular upgrades enable western blotting, imaging cytometry and injectors. The SpectraMax i2X is used for cell migration, cell. 価格、見積もり、お近くの販売店を. 316 grating motor driver faulty. HTRF IP1 standard curve measured on the SpectraMax i3x (green circles) and SpectraMax iD5 (red circles) readers. The SpectraMax ® i3x reader provides the. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. SpectraMax i3x Multi-Mode Microplate Reader SystemSpectraMax® i3x dari Molecular Devices adalah sistem deteksi berbasis multi-mode yang berevolusi menyesuaik. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Enzyme activity was presented as absolute activity in a unit of nmol g −1 dry soil h −1. 303 unable to cal dark current. The size and fluorescence intensity were tested for 48 h. 2 software (Stat-Ease) to identify factors that have significant effects on cell growth or IgG production, either alone or in combination with. TR-FRET was measured on the SpectraMax i3x and iD5 readers (see Table 1 for instrument settings). In dual reporter gene assays, cells are transfected with two vectors, the first containing an experimental reporter gene coupled to a regulated promoter of interest, andSpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Microplate Stacker. Simplified workflow: Add cellular imaging assays in a small footprint. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. Instrument. With a preconfigured protocol to simplify data acquisition and analysis, SoftMax Pro Software completes this total solution for fast, sensitive, and reliable dual luciferase reporter assay results. 2C). About the reader setup recommendations The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. This assay uses two fluorescent dyes, calcein AM (cal AM) and ethidium. 4. The SpectraMax i3x Injector Cartridge with SmartInject Technology is a data sheet that describes the features and benefits of this innovative accessory for the SpectraMax i3x Multi-Mode Detection Platform. • SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . Animal imaging assay was performed on a IVIS R Lumina Series III small animal optical system.